AAV Helper-Free System，AAV Helper-Free System說明書，AAV Helper-Free System載體

pAAV-IRES-hrGFP

AAV Helper-Free System （Catalog #240071） 

Materials Provided Concentration Quantity 

pAAV-MCS vector 1 μg/μl in TE buffer 10 μg 

pCMV-MCS vector 1 μg/μl in TE buffer 10 μg 

pAAV-LacZ vector 1 μg/μl in TE buffer 10 μg 

pAAV-RC plasmid （sufficient for two transfections） 1 μg/μl in TE buffer 20 μg 

pHelper plasmid （sufficient for two transfections） 1 μg/μl in TE buffer 20 μg 

AAV-293 cells （Catalog #240073） — 1 × 106 cells 

AAV-HT1080 cells （Catalog #240109） — 1 × 106 cells 

pAAV-hrGFP （Catalog #240074） 

Materials Provided Concentration Quantity 

pAAV-hrGFP control plasmid 1 μg/μl in TE buffer 20 μg 

pAAV-IRES-hrGFP （Catalog #240075） 

Materials Provided Concentration Quantity 

pAAV-IRES-hrGFP vector 1 μg/μl in TE buffer 10 μg