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Cell Tech FLNADH 100 說(shuō)明書(shū)

更新時(shí)間：2017-08-02 點(diǎn)擊次數(shù)：4644

世界*實(shí)驗(yàn)材料供應(yīng)商 Cell Technology正式授權(quán)上海起發(fā)為其中國(guó)代理， Cell Technology在一直是行業(yè)的*,一直為廣大科研客戶(hù)提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國(guó)科研客戶(hù)帶來(lái)的產(chǎn)品，的服務(wù)，簽約 Cell Technology就是為了給廣大科研客戶(hù)帶來(lái)更加完善的產(chǎn)品和服務(wù),您的滿(mǎn)意將是我們zui大的收獲

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Cell Technology成立于1998年，致力于為研究院、生物技術(shù)公司及制藥企業(yè)的科研人員研發(fā)生產(chǎn)*的細(xì)胞功能研究用試劑與試劑盒。這些檢測(cè)試劑與試劑盒可以實(shí)現(xiàn)不破壞細(xì)胞形態(tài)和結(jié)構(gòu)的情況下原位檢測(cè)細(xì)胞成分，研究細(xì)胞內(nèi)發(fā)生的特定事件.Cell Technology發(fā)布了通過(guò)測(cè)定胞內(nèi)活性caspase及線粒體膜電位研究細(xì)胞凋亡的一系列試劑。隨著公司研發(fā)團(tuán)隊(duì)的持續(xù)努力，我們又推出了一系列高特異性的caspase檢測(cè)試劑，以及cathepsin檢測(cè)的試劑和ELISA試劑盒。目前Cell Technology提供的檢測(cè)試劑和ELISA試劑盒涵蓋Caspase、NOS、ROS、線粒體膜電位、信號(hào)通路和蛋白磷酸化等多個(gè)研究領(lǐng)域，產(chǎn)品倍各大高校、研究機(jī)構(gòu)、生物技術(shù)企業(yè)和制藥企業(yè)采用。

Cell Technology主要產(chǎn)品簡(jiǎn)介

1、Fluoro Cholesterol：高靈敏、一步檢測(cè)生物樣本中總膽固醇含量，可用于細(xì)胞代謝與藥物作用的研究。

2、Fluoro NADP：檢測(cè)組織或細(xì)胞提取液內(nèi)NADP/NADPH含量，與NAD/NADH檢測(cè)無(wú)交叉，可用于細(xì)胞死亡、抗氧化/氧化應(yīng)激、能量代謝及線粒體功能研究。

3、Fluoro Sarcosine：定量檢測(cè)細(xì)胞、血清及組織提取物中的肌氨酸含量。

4、Fluoro IndoBlu：定量檢測(cè)細(xì)胞中IndoBlu的含量，適用于細(xì)胞增殖研究。

5、Fluoro Lactate：定量檢測(cè)生物樣本中 L-lactate的含量。

6、Fluoro Phosphate：定量檢測(cè)生物樣本中的磷含量，靈敏度可達(dá)200nM。

產(chǎn)品編號(hào):FLNADH 100-2
產(chǎn)品名稱(chēng):Fluoro NAD™ Fluorescent NAD/NADH Detection Kit - Detection of NAD/NADH in tissue extracts /cell samples/熒光NAD/NADH檢測(cè)試劑盒
產(chǎn)品規(guī)格:100 Tests

Fluoro NAD™ NEW!
Fluorescent NAD/NADH Detection Kit - Detection of NAD/NADH in tissue extracts /cell samples

Key Benefits:

Detection of NAD/NADH activity in cells or tissue extracts
Detection of NAD/NADH levels in apoptosis, metabolism, proliferation, DNA repair, senescence, endocrine signaling and life span.
NAD/NADH detection in Bacterial, fungal and plant cells.
Fluorescent 96 well Plate Reader Readout (excitation: 530-570nm and emission at 590-600nm)

Introduction

The role of pyridine nucleotides (NAD/NADH) metabolism in health, is of continual and increased interest. A growing amount of evidence supports the fact that NAD metabolism regulates important biological effect including life span. NAD, through poly-ADP-ribosyl polymerase (PARP), mono-ADP-ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation and are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling. This suggests that either the enzymes or NAD could be an important therapeutical target ¹.

Assay Principle

The Fluoro NAD/NADHdetectionkit utilizes a non-fluorescent detection reagent, which is oxidized in the presence NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction. The enzyme reaction specifically reacts with NAD/NADH and not with NADP/NADPH.

Reaction:

1. NADH + non-fluorescent detection reagent+ electron coupler -----> fluorescent
analog + NAD

2. NAD + enzyme coupled reaction -------> NADH (then proceeds to reaction 1).

excitation: 530-570nm and emission at 590-600nm

References

Anthony A. Sauve NAD⁺ and Vitamin B3: From metabolism to therapies
J. Pharmacol. Exp. Ther. 2007 : jpet.107.120758v1

Kit contents (for 100 assays)

Enzyme Mix ..................................................Part# 6021 - Store at -20C
NADH Detection Reagent .............................Part# 4018 - Store at -20C
NADH Standard .............................................Part# 7013 - Store at -20C
NAD/NADH Reaction Buffer ........................Part# 3044 - Store at +2-8 C
NAD/NADH Lysis Buffer ..............................Part# 3045 - Store at +2-8 C
NAD Extraction Buffer ..................................Part# 3046 - Store at +2-8 C
NADH Extraction Buffer ................................Part# 3047 - Store at +2-8 C
NADH Standard Diluent ................................Part# 3051 - Store at +2-8 C

The following kits are available:
Catalog #	Size	Price (RMB)
FLNADH 100-2	100 Tests	5250.00

我們公司zui大優(yōu)勢(shì)是強(qiáng)大的采購(gòu)，

1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，

2：貨品全，現(xiàn)經(jīng)營(yíng)過(guò)700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢(shì)，

3：提供加急服務(wù)，一般1-2周到貨，超過(guò)時(shí)限加急費(fèi)全免

4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢(shì)，當(dāng)然不能保證100%產(chǎn)品都是,因?yàn)橐馕吨鴽](méi)有服務(wù).

5：良好的信譽(yù)，大部分客戶(hù)我們提供貨到付款服務(wù)，客戶(hù)包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國(guó)藥，fisher等500多家公司

6：我們*代理的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc. Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，QA-Bio,Inc，QA-Bio,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等

7：我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發(fā)，歡迎合作。

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